The Joslin Flow Cytometry Core provides reliable and affordable cell sorting and flow cytometry services to its users so that they can isolate, analyze, and study cells that increase our understanding of diabetes and its complications, and ultimately develop treatments and cures for these diseases.
Cell sorting technology is continuously evolving and improving, enabling new approaches to questions in diabetes research. In addition, because flow cytometry is traditionally thought of as an immunology tool, the Joslin Core’s mission includes activities that enhance the use of flow cytometry in other research areas, bringing this technology to new users and stimulating new avenues of diabetes research.
Services1. Cell analysis
- BD LSR-II: This instrument is equipped with four laser lines (488nm, 405nm, 561nm, and 640nm) and 15 fluorescent detectors. The current configuration allows for 2 colors off the blue laser, 5 colors off the yellow-green laser, 3 colors off the red, and 6 off of the violet.
- Miltenyi Biotec MACSQuant: The MACSQuant is capable of detecting up to 8 fluorescent parameters and is equipped with 488nm, 633nm, and 405nm solid-state lasers. This instrument has the ability to provide absolute cell counts.
- BD LSRFortessa: This instrument is equipped with five laser lines (445nm, 405nm, 488nm, 561nm, and 640nm) and 17 fluorescent detectors, and the HTS (High Throughput Sampler) system which will enable fully automated sample acquisition from 96- and 384-well microtiter plates. The current configuration allows for 2 colors off the 445nm laser, 3 colors off the blue laser including GFP and YFP detection, 5 colors off the yellow-green laser, 3 colors off the red, and 4 off of the violet. (Summer of 2016)
2. Cell sorting
- MoFlo Legacy: This cell sorter is capable of high speed and single cell sorting on up to 8 fluorescent parameters and is equipped with 3 lasers (488nm, 355nm and 640nm). The instrument has the capability of four-way sorting into tubes or single cell sorting directly into plates or slides.
- SORP BD FACSAria: This instrument is capable of high speed and single cell sorting on up to 15 fluorescent parameters and is equipped with 5 lasers (355nm, 488nm, 405nm, 561nm, and 640nm). It is capable of four-way sorting into tubes or single cell sorting directly into plates or slides. This instrument is equipped with an Aerosol Containment System for the sorting of BSL-2 biohazardous samples.
- BD FACSAria IIu: This self-run instrument is capable of high speed and single cell sorting on up to 12 fluorescent parameters and is equipped with 3 lasers (488nm, 405nm and 640nm). It is capabile of four-way sorting into tubes or single cell sorting directly into plates or slides. This instrument is also equipped with an Aerosol Containment System for the sorting of BSL-2 biohazardous samples.
Consult the facility personnel for fluorochrome combination feasibility and necessary controls prior to designing your experiment.
3. Large object sorting
- COPAS: The Complex Object Parametric Analyzer and Sorter (COPAS) BioSort system is specially designed to analyze and sort objects ranging from 40-250 microns.
4. Magnetic cell enrichment
- AutoMACS: The AutoMACS provides high yield enrichment of rare cell populations via magnetic selection, which helps to reduce per user sort times by enabling better pre-sort enrichment of target cells.
5. EliSpot Reader: This analyzer is designed for scanning and evaluating a wide range of microtiter plate-based bioassays—in particular, ELISPOT assays. This instrument offers:
- Uncompromised quality of analyzer components
- Precise, fast, user-friendly scanning
- Objective, reproducible counting results
6. Automatic cell counting
- Cellometer: The Cellometer Vision Trio Cellprofiler system combines bright field microscopy and fluorescence detection to determine total cell numbers, measure cell viability, and quantify GFP expression.
- Hemavet: The Hemavet 950FS Multi Species Hematology System specializes in counting differential white blood cells (WBCs) with 24 parameters including total WBC, erythrocyte, lymphocyte, monocyte, neutrophil and other granulocyte subtypes, and platelet numbers.
7. Data analysis: For flow cytometry data analysis the Core provides 3 FlowJo dongles that can be signed out from the core on a weekly basis.
Effective January 1, 2014:
|Joslin/HSCI/DRC affiliates||||||Non affiliates|||||
|Instrument||Staff Run||Self-Run||Instrument||Staff Run||Self-Run|
|Aria - Dobby||$45.00/hr||N/A||Aria - Dobby||$100.00/hr||N/A|
|Aria - Flo||N/A||$35.00/hr||Aria - Flo||N/A||$70/hr|
|COPAS|| || ||COPAS|| || |
|LSR II||N/A||$35.00/hr||LSR II||N/A||$70.00/hr|
|Training for Aria, LSR II and MQuant :- $45.00/hr||||||Training for Aria, LSR II and MQuant :- $100.00/hr|||||
All sorting cancellations (either self-run or operator assisted) require 48 hours' notice. To cancel time with less than 48 hours' notice, you will need to contact the Joslin Flow Core staff to cancel the time on your behalf.
All analysis cancellations require 24 hours' notice. To cancel time with less than 24 hours' notice, you will need to contact the Joslin Flow Core staff to cancel the time on your behalf.
To book time less than 24 hours prior to the start time, you will need to contact the Joslin Flow Core staff to have them book the time on your behalf.
The minimum charge for cell sorters is one hour and for analyzers is ½ hour.
On all instruments, users are charged for the entirety of their reservation time. This means that if you are late you will be charged for the time between your scheduled start and your actual arrival. It also means that if you finish early, you will still be charged for the remainder of the scheduled time.
No-shows will incur usage fees for the entire reservation.
Cancellation deadlines are 48 hours before the start of any sorting appointment and 24 hours before the start of any analysis appointment. You must cancel your appointment in the Cortex Application Suite by these deadlines.
Cancellations made after the deadlines are treated as a no-show. You will be charged the full usage fees for your late cancellation unless someone else schedules for the canceled time.
Facility users are billed every month for hourly usage.
Hours of Operation
9.00 am – 5.00 pm Monday, Tuesday, Wednesday and Friday; 1.00 pm – 8.00 pm on Thursday
Trained and experienced users may use the self-run instruments outside of the Core's staffed hours.
For questions about the flow cytometry core and its services, please contact Girijesh.Buruzula@joslin.harvard.edu